Journal: Bioresources and bioprocessing

Article Title: Effect of Akkermansia muciniphila on pancreatic islet β-cell function in rats with prediabetes mellitus induced by a high-fat diet.

doi: 10.1186/s40643-024-00766-4

Figure Lengend Snippet: Fig. 2 Effects of A. muciniphila treatment and dietary intervention on GSIS, apoptosis and differentiation in the β cells of rats with pre-DM: A GSIS index in the β cells of rats in different groups; B TUNEL and insulin immunofluorescence staining (×400) of the pancreatic tissues of rats in different groups (blue fluorescence is nuclear dye [DAPI] staining, red fluorescence represents insulin-positive β cells, green fluorescence represents TUNEL-positive apoptotic cells, and Merge is TUNEL combined with insulin immunofluorescence staining); C Comparison of the percentage of TUNEL+ β cells of rats in different groups; D-I Comparison of the expression of Bcl-2, Bax, Bcl-2/Bax, Caspase3, MAFA and MAFB at the mRNA level in the islets of rats in different groups; J Comparison of the expression of MAFA and MAFB at the protein level in the islets of rats in different groups. Different letters in the same bar represent significant differences between the treatments when P < 0.05

Article Snippet: with protein-free rapid blocking buffer (PS108P, Epizyme Biotech, Shanghai, China) at room temperature for 1 h, the membranes were incubated with different primary antibodies at 4 °C overnight, such as a MAFA rabbit monoclonal antibody (mAb) (1:1000, 79,737, Cell Signaling Technology, Danvers, MA, USA), a MAFB rabbit mAb (1:1000, 30,919, Cell Signaling Technology, Danvers, MA, USA), an Occludin mAb (1:1000, 33-1500, Invitrogen, Carlsbad, CA, USA), an anti-TLR2 antibody (1:500, ab209217, Abcam, Cambridge, UK), an anti-TLR4 antibody (1:500, ab13867, Abcam, Cambridge, UK), a GAPDH rabbit mAb (1:5000, 5174, Cell Signaling Technology, Danvers, MA, USA), and a beta actin polyclonal antibody (1:5000, 20536-1-AP, Proteintech, Chicago, IL, USA).

Techniques: TUNEL Assay, Immunofluorescence, Staining, Fluorescence, Comparison, Expressing